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Safety data from animal studies,
Steroids & Metal free Certified.
The Toxological Studies, Storage
Stability Studies and are being
successfully made from the FDA Approved
Lab. These medicines proved to be free
from any inclusion of Steroids or Heavy
Metals.
Also the
Certificate of test or analysis by
Government analyst and from Drug Control
Laboratory Maharashtra State, Mumbai,
reveals the safety of the drugs against
all metal, steroids, chemicals and other
harmful ingredients.
For better understanding of the drugs
and its effective action against HIV
virus one should undergo the below
mentioned testing and undertake the
studies from any renowned &
well-equipped institution.
1) ARV Testing or Drug Sensitivity
Testing to realize the inhibition rate
of the drug against HIV.
2) HIV Phenotyping test to realize that
the drug is completely free from
multi-drug resistance.
3) Invivo Clinical studies to realize
the improvement in CD4 cells & overall
health of the patients.
4) RNA viral load / Realtime RT-PCR
Quantitative testing to evaluate the
rate of decrease in viral load in the
patients in the 1st month, 2nd month
and 3rd month of the treatment.
INVITRO STUDIES:
In invitro lab studies, this drug was
put for Drug Sensitivity Test and
Anti-retroviral Activity Test in the
laboratory adhering to the standard
protocol and the highest inhibition rate
of 98% was recorded against the
causative agent, HIV virus. The detail
procedures undertaken in an invitro lab
study are mentioned as under: -
Procedure of the invitro lab study
-
Collect 20 ml Blood in an EDTA
containing vial
-
Mix it properly and keep at room
temperature
-
Overlay this blood sample on 2 ml
Histopaque solution in
25-ml sterile centrifuge tube.
Centrifuge the tube at 1500 rpm for
20 min
-
Two layers will be formed after
centrifugation
-
Separate out the upper layer of
Histopaque with the help
of sterile pipette and collect the
lymphocyte ring in 2 ml sterile
eppendorf tube
-
Microfuge it for 1 min at the bottom
of the eppendorf tube lymphocytes
sediment will be observed.
-
Re-suspend the sediment in 2-ml
fresh RPMI-C Media.
-
Prepare the serial dilutions of the
drug of our interest or to be tested
in the range of 1ug/ml – 500 ug/ml
-
Add 500 ul of the lymphocyte culture
with the help of sterile pipette /
tips and add the appropriate
dilution of the drug to the
respective culture well along with
control without addition of any
drug.
-
Incubate it in CO2 incubator at 37
degree Celsius (5% CO2)
-
After incubation do the P-24 ELISA
test for Control and for Test
-
Interpret the results on the basis
of difference in between the Control
O.D and Test O.D and calculate the %
Inhibition of the viral growth in
Test in comparison with Control.
-
O.D of the Control always ranges
from 0.2-0.4 while the O.D of Test
ranges in between 0.06-0.1, which
indicates the inhibition of the
virus multiplication.
INVIVO STUDIES:
In the Invivo human clinical studies,
this drug were provided as a treatment
to the HIV patients through HAKH Medical
Foundation and the improvements in the
patients in terms of physical as well as
pathological were recorded in proper,
controlled condition through standard
procedure and all the blood test
pertaining to HIV were undertaken
through Molecular Diagnostic Services in
association with Reliance Life Science
and the reports & blood samples were
maintained for more than 3-5 yrs for
repeatability.
The clinical observation of this drug
revealed the following improvements
in the patients:
-
7-8 folds decrease in HIV viral load
counts within 5-6 weeks
of the treatment.
-
Viral load count decreased to less
than detectable limit (>100)
within 5-7 months of the treatment.
-
Augment in CD4 count noted.
-
4-6 pounds increment in weight
within 2-4 months.
-
Relief from HIV symptoms.
-
Prophylactic against opportunistic
infections.
Phenomenal change in DNA-PCR Antigen
test
Patients those continued the treatment
for 18-20 months after attaining the
viral load to less than detectable limit
and adhering to instruction strictly had
recorded HIV DNA-PCR Antigen Qualitative
test ’Not Detected’ and the test
interpreted ‘the blood sample to be
negative for the presence of HIV virus”.
And so the medication were completely
stopped for almost one year and again
undergone for HIV DNA-PCR Antigen test
(Qualitative) and recorded ‘Not
Detected’. The same test was repeated
for 2nd, 3rd & 4th year after completely
stopping the medications and the
patients recorded ‘Not Detected’ for the
test.
The method for the confirming the
presence of HIV antigen is HIV DNA-PCR
Antigen test (Qualitative) test by
Nested PCR Technique.
A major
breakthrough on HIV research through
Antigen Vs
Antibody Test.
HIV antibodies remain in the body for a
long time to come although HIV antigen
is completely evaded from the body. This
Methods: 6ml serum sample is to be drawn
from the HIV patients who were earlier
confirmed HIV positive through Western
blot test and later undergone complete
treatment with Unani medicine HOO-IMM
PLUS. The sample is to be cautiously
handled as per the NABL guidelines and
to be sent to a renowned and reliable
PCR Molecular diagnostic lab. 3 ml of
the sample is to be allocated for
Western Blot Antibody test and the other
3 ml for HIV DNA-PCR Antigen test.
Results: Our study showed that the above
mentioned tests showed that the blood
sample is positive for Western blot
Antibody test & Negative for HIV DNA-PCR
Antigen test. This directly interprets
that at present the person is HIV
negative though he still carries HIV
antibodies authenticating his earlier
infection with HIV virus.
Conclusion: It could be concluded that
our body immune system creates cell
mediated immunity responses towards HIV
antigen to produce antibodies that may
persist for a long time, even after the
antigen is completely eliminated from
the body. The other best example is
Mycobacterium Tuberculosis antibodies.
The human body can respond to antigen in
many different ways. These fall into two
major categories:
• Antibody-mediated immunity.
Antibodies, dissolved in blood, lymph,
and other body fluids bind the antigen
and trigger a response to it. (This form
of immunity is also called humoral
immunity).
• Cell-mediated immunity (CMI). T cells
(lymphocytes) bind to the surface of
other cells, display the antigen and
trigger a response and may remain in the
body for a long time. The response may
involve a) other lymphocytes and b) any
of the other white blood cells
(leukocytes)
EVIDENTIAL VALIDATION:
The foolproof evidence for the proven
efficacy of our herbal medicine HOO- IMM
Plus is as elucidated below: -
1) 6ml serum / blood sample is to be
drawn from a person previously infected
with HIV/AIDS and undergone the complete
course of treatment with our medicine
HOO-IMM Plus.
2) The sample is to be well handled as
per the NABL guidelines and to be sent
to a renowned and reliable PCR Molecular
diagnostic lab.
3) 3 ml of the sample is to be put for
Western Blot Antibody test and the other
3 ml for HIV DNA-PCR Antigen test.
4) The result of the above mentioned
tests would report that the blood sample
is positive for Western blot Antibody
test & Negative for HIV DNA-PCR Antigen
test.
5) This Interprets that at present the
person is HIV negative though he still
carries HIV antibodies (which is not
harmful to health), authenticating his
earlier infection with HIV virus.
6) The scientific reason behind this
reporting is that our body immune system
creates cell mediated immunity responses
towards HIV antigen to produce
antibodies that may persist for a long
time, even after the antigen is
completely eliminated from the body. The
other best example is Mycobacterium
Tuberculosis antibodies.
The human body can respond to antigen in
many different ways. These fall into two
major categories:
· Antibody-mediated immunity.
Antibodies, dissolved in blood, lymph,
and other body fluids bind the antigen
and trigger a response to it. (This form
of immunity is also called humoral
immunity).
· Cell-mediated immunity (CMI). T cells
(lymphocytes) bind to the surface of
other cells, display the antigen and
trigger a response and may remain in the
body for a long time. The response may
involve a) other lymphocytes and b) any
of the other white blood cells
(leukocytes)
7) Thus the above mentioned persons who
are free from HIV antigen can, now
freely marry and indulge in sexual
activities without any risk of infection
to their life partner, women can
conceive without any chance of
transmitting the disease to children
(though antibodies are likely to be
transmitted between the marital partners
or from mother to fetus, it is not
harmful to health) and lead a very
normal life.
(It seems that many of the laboratories
and doctors are still unaware of this
latest molecular diagnostic test) |
